Olympus TIRF Specifications
This Total Internal Reflection Fluorescence (TIRF) instrument is designed for high-speed acquisition of structures in close proximity to the glass-water interface. Note that temperature control is not yet fully supported.
| Microscope | |
| Olympus IX81 microscope (inverted) | |
| Objective | |
| 60x PlanApo-N 1.45 NA, oil | |
| Fluorescence Filters Cubes (visual inspection only) | |
| FITC / GFP: 450-490 nm excitation, 515-586 nm emission. | |
| Rhodamin: 540-552 nm excitation, emission beyond 590 nm. | |
| DAPI: 350-400 nm excitation, 417-477 nm emission. | |
| Laser Lines | |
| 458, 488, 514 nm: Argon ion laser, 200 mW | |
| 568, 647nm: Argon-Krypton ion laser, 81 mW | |
| AOTF switched for rapid switching | |
| Detector | |
| Hamamatsu Orca C9100 backed-thinned camera, 512x512 pixels | |
| Computer & Software | |
| Macintosh G4 800 MHz 1.5GB ram | |
| OpenLab acquisition package | |
