Olympus BX61 Specifications
Confocal microscopy is not always the best solution to your imaging needs and, in some situations, may even be counter-productive. The fully motorized Olympus BX61 is a computer-controlled conventional wide-angle (non-confocal) upright microscope. Equipped with a motorized stage and a high-resolution digital camera, this platform allows for maximum flexibility in excitation and emission wavelengths with dual filter wheels. High-quality Phase and Differential Interference Contrast are fully supported. Live cell imaging is available either via water-immersion objectives or a Bioptechs FCS-3 incubation chamber (requires growing cells on special coverslips).
| Microscope | |
| Olympus BX61 (Upright) | |
| Motorized nosepiece, filter wheel and focus | |
| Prior ProScan II Motorized XY stage | |
| Objectives | |
| 10x UplanFL air Ph1, 0.3 NA | |
| 20x UplanFL air Ph1, 0.5 NA | |
| 40x Uplan FL oil DIC | |
| 60x PlanApo oil DIC, 1.42 NA | |
| 20x UApo water DIC, 0.75 NA | |
| 60x UPLSAPO water DIC, 1.2 NA | |
| Fluorescence Filters Cubes | |
| #1: DAPI (Ex: 350/50, Dichroic: 400. Em: 460/50 nm) | |
| #2: FITC (Ex: 470/40, Dichroic: 495, Em: 525/50 nm) | |
| #3: TRITC (Ex: 545/30, Dichroic: 570, Em: 620/60 nm) | |
| #4: Dual dichroic CFP / YFP (No visual, 455-485 and 515-568 nm) | |
| Illumination System: | |
| Sutter Instruments Lambda 10-3 excitation & emission filter wheels. Because the emission filters are located before the camera, no visual observation is possible. | |
| Excitation Filter Wheel Positions | |
| #1: OPEN | |
| #2: BLOCKED | |
| #3: CFP exciter 430/24 nm | |
| #4: YFP exciter 500/20 nm | |
| #5: BLOCKED | |
| #6: BLOCKED | |
| #7: BLOCKED | |
| #8: BLOCKED | |
| #9: BLOCKED | |
| #10: BLOCKED | |
| Emission Filter Wheel Position | |
| #1: OPEN | |
| #2: BLOCKED | |
| #3: CFP emitter 470/24 nm | |
| #4: YFP emitter 535/30 nm | |
| #5: BLOCKED | |
| #6: BLOCKED | |
| #7: BLOCKED | |
| #8: BLOCKED | |
| #9: BLOCKED | |
| #10: BLOCKED | |
(Additional filters and dichroics may be purchased and installed for a nominal cost) |
|
| Camera | |
| Photometrics CoolSnap FX cooled CCD,12-bit sampling, 1300 x 1030 pixels, 20MHz digitization, flexible binning. | |
| Computer and Software | |
2.6GHz Pentium 4 CPU running Windows XP Professional, 2.0 GB RAM, dual 95 GB HD storage, 8x DVD-RW drive, 21” Samsung 1600 x 1200 flat panel monitor |
|
Universal Imaging Corp. MetaMorph (v. 6.3.7) image acquisition & analysis software with Multi Dimensional Acquisition (MDA) module. |
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