Perkin Elmer Ultraview RS Specifications
The Ultraview RS is an Instrument optimized for high-speed acquisition for live cells imaging. Equipped with a dual-disk Nipkow confocal scanner and a piezo-electric focusing mechanism, the platform may achieve scan rates up to 100 frames per second on bright specimens (realistically, the actual scan rates may be limited by light throughput).
| Microscope | |
| Zeiss Axiovert 200 M microscope (Inverted) | |
| Objectives | |
| 10x Zeiss plan-apochromat dry, 0.45 NA | |
| 20x Zeiss plan-neofluar dry, 0.50 NA | |
| 40x Zeiss plan-neofluar dry, 0.75 NA | |
| 63X Zeiss plan-apochromat oil, 1.4 NA | |
| 100x Zeiss plan-neofluar oil, 1.30 NA | |
| Fluorescence Filters Cubes (visual inspection only) | |
| FITC / GFP: 450-490 nm excitation, 515-586 nm emission. | |
| Rhodamin: 540-552 nm excitation, emission beyond 590 nm. | |
| DAPI: 350-400 nm excitation, 417-477 nm emission. | |
| Laser Lines (scanning only) | |
| Argon-Krypton: 488, 568 and 647 nm | |
| AOTF switched (custom ROI, FRAP and FRET not supported) | |
| Filter blocks (Scanning only) | |
| FITC: 488 nm excitation, emission beyond 505 nm | |
| FITC/Rhodamin/Cy5: triple excitation, band-pass emission on each channel (This filter has a poor efficiency and may severely limit the acquisition rates) | |
| Detector | |
| Cooled Hammamatsu Orca-ER CCD camera, 12-bit, 1344 x 1024 pixels, better than 33 frames per second (light throughput allowing). | |
| Computer & Software | |
| Dell Workstation dual 2.4 GHz Xeon CPU running Windows 2000, 2GB RAM, dual 11GB hard drives, 24 inch Samsung flat panel monitor. | |
| Perkin-Elmer UltraView ERS acquisition software, version 1.0016 | |
| Volocity by Improvision for 3D Rendering software, version 3.0.2 | |
