| This point scanning instrument is available for either fixed slides or live specimen with temperature control, perfusion and solution changes. Photoactivation (405 nm), FRAP and FRET are fully supported. A Meta detector allows for spectral imaging and online emission fingerprinting. | ||
| Microscope | ||
| Zeiss Axiovert 200 M microscope (inverted) | ||
| Objectives | ||
| 10x Zeiss plan-neofluar dry, 0.3 NA | ||
| 20x Zeiss plan-apochromat dry, 0.75 NA | ||
| 40x Zeiss plan-neofluar oil, 1.3 NA, DIC | ||
| 63X Zeiss plan-apochromat oil, 1.4 NA, DIC | ||
| 100x Zeiss alpha plan fluar oil, 1.45 NA, DIC | ||
| Fluorescence Filters Cubes (visual inspection only) | ||
| FITC / GFP: 450-490 nm excitation, 515-586 nm emission. | ||
| Rhodamin: 540-552 nm excitation, emission beyond 590 nm. | ||
| DAPI: 350-400 nm excitation, 417-477 nm emission. | ||
| Cy5: 610-650 nm excitation, 670-720 nm emission. | ||
| Laser Lines (scanning only) | ||
| 405 nm: solid-state laser, 25 mW | ||
| 458, 488 and 514 nm: Argon ion laser, 30 mW | ||
| 543 nm: Helium-Neon laser, 1mW | ||
| 633 nm: Helium-Neon laser, 15mW | ||
| AOTF switched: photoactivation, FRAP, FRET and uncagging fully supported | ||
| Filter Blocks (scanning only, must be used in certain combinations) | ||
| Excitation: | ||
| NT 80/20: beam splitter, 20% excitation reflected, 80% emission transmitted | ||
| HFT 405/488/543/633: dichroic 405, 488, 543 and 633 nm | ||
| HFT 405/488/543: dichroic 405, 488 and 543 nm | ||
| HFT 405/514: dichroic 405, 514 nm | ||
| HFT 458/514: dichroic 458, 514 nm | ||
| HFT 458/543: dichroic 458, 543 nm | ||
| HFT 458: dichroic 458 nm | ||
| HFT 488: dichroic 488 nm | ||
| Steering Meta vs PMT2/3: | ||
| NFT 490: long pass 490 nm | ||
| NFT 515: long pass 515 nm | ||
| NFT 545: long pass 545 nm | ||
| NFT 635 vis: long pass 635 nm | ||
| NFT KP 545: short pass 545 nm | ||
| Plate: glass plate correcting for misalignment, automatically inserted by software | ||
| Steering PMT 2 vs. PMT 3: | ||
| NFT 490: long pass 490 nm | ||
| NFT 515: long pass 515 nm | ||
| NFT 545: long pass 545 nm | ||
| Emission Filters | ||
| Meta detector | ||
| The Meta is a spectrometer spreading fluorescence emission among an array of detectors. It is, in itself, a configurable emission filter. | ||
| PMT 2 | ||
| LP 420: long pass 420 nm | ||
| LP 475: long pass 475 nm | ||
| LP 505: long pass 505 nm | ||
| BP 420-480 IR: band pass 420 to 480 nm, IR-blocking | ||
| BP 470-500: band pass 470 to 500 nm | ||
| BP 475-525: band pass 475 to 525 nm | ||
| BP 505-530: band pass 505 to 530 nm | ||
| PMT 3 | ||
| LP 505: long pass 505 nm | ||
| LP 560: long pass 560 nm | ||
| LP 650: long pass 650 nm | ||
| BP 500/20 IR: band pass 490 to 510 nm, IR-blocking | ||
| BP 505-530: band pass 505 to 530 nm | ||
| BP 505-550: band pass 505 to 550 nm | ||
| BP 530-600: band pass 530 to 600 nm | ||
| BP 560-615: band pass 560 to 615 nm | ||
| Detectors | ||
| Two photo-multiplier tubes fluorescence, 8 or 12 bit sampling (256 or 4096 gray levels, respectively) | ||
| One transmission detector for transmission / DIC imaging | ||
| One Meta detector for spectral imaging.. Up to 8 channels may be defined, each grouping any of the 32 detectors with a spectral width of 10 nm each (overlap between channels is not allowed). The meta detector is almost as sensitive as a PMT except at higher wavelength. However, its intrinsic noise is twice as high as a PMT. | ||
| Computer & Software | ||
| Fujitsu Siemens 2.4 GHz Xenon CPU running Windows 2000, 4 GB RAM, dual 114 GB storage, 2.4x DVD+/- recorder, dual 19" 1280 x 1024 flat panels | ||
| LSM 5 acquisition software with Physiology and Time Series modules | ||
