| Equipped with water-immersion lenses, an infra-red excitation source and a highly sensitive non-descanned detector, this instrument is oriented toward thick tissue sections and live animal imaging. A Meta detector allows for spectral imaging and online emission fingerprinting. | ||
| Microscope | ||
| Zeiss Axioskop 2 FS microscope (upright) | ||
| Objectives | ||
| 5x Zeiss plan-apochromat dry, 0.16 NA | ||
| 10x Zeiss achroplan water, 0.3 NA | ||
| 20x Zeiss achroplan water, 0.5 NA | ||
| 40x Zeiss achroplan, water, 0.8 NA, IR, DIC | ||
| 63X Zeiss achroplan water, 0.9 NA, IR, DIC | ||
| Note: only two objectives are available on the microscope at any given time. Swapping objectives also requires software adjustment. Contact MIC staff. | ||
| Fluorescence Filters Cubes (visual inspection only) | ||
| FITC / GFP: 450-490 nm excitation, 515-586 nm emission. | ||
| Rhodamin: 540-552 nm excitation, emission beyond 590 nm. | ||
| DAPI: 350-400 nm excitation, 417-477 nm emission. | ||
| Note: the filter wheel also contains a cube used for IR excitation and non-descanned imaging. The DAPI cube may be swapped with the polarizer required for DIC imaging | ||
| Laser Lines (scanning only) | ||
| 458, 488 and 514 nm: Argon ion laser, 30 mW | ||
| 543 nm: Helium-Neon laser, 1mW | ||
| 633 nm: Helium-Neon laser, 15mW | ||
| 720-930 nm: tunable Chameleon IR laser, 1,870 mW max (2-photon excitation) | ||
| AOTF switched: FRAP, FRET and uncagging fully supported | ||
| Filter Blocks (scanning only, must be used in certain combinations) | ||
| Excitation: | ||
| NT 80/20: beam splitter, 20% excitation reflected, 80% emission transmitted | ||
| HFT UV/488/543/633: dichroic UV, 488, 543 and 633 nm | ||
| HFT KP 700/488: excitation above / emission below 700 nm + 488 nm dichroic | ||
| HFT KP 700/543: excitation above / emission below 700 nm + 543 nm dichroic | ||
| HFT 458/514: dichroic 458 and 514 nm | ||
| HFT 458: dichroic 548 nm | ||
| HFT 488: dichroic 488 nm | ||
| HFT KP 650: excitation above 650 nm, emission below 650 nm | ||
| Steering Meta vs PMT2/3: | ||
| NFT 490: long pass 490 nm | ||
| NFT 515: long pass 515 nm | ||
| NFT 545: long pass 545 nm | ||
| NFT 635 vis: long pass 635 nm | ||
| NFT KP 545: short pass 545 nm | ||
| Plate: glass plate correcting for misalignment, automatically inserted by software | ||
| Steering PMT 2 vs. PMT 3: | ||
| NFT 490: long pass 490 nm | ||
| NFT 545: long pass 545 nm | ||
| BG 39: IR blocking plate | ||
| Emission Filters | ||
| Meta detector | ||
| The Meta is a spectrometer spreading fluorescence emission among an array of detectors. It is, in itself, a configurable emission filter. | ||
| PMT 2 | ||
| KP 685: short pass 685 nm (IR laser) | ||
| BP 390-465 IR: band pass 390 to 465 nm, IR-blocking | ||
| BP 435-485 IR: band pass 435 to 485 nm, IR-blocking | ||
| BP 480-520 IR: band pass 480 to 520 nm, IR-blocking | ||
| BP 500-530 IR: band pass 500 to 530 nm, IR-blocking | ||
| BP 500-550 IR: band pass 500 to 550 nm, IR-blocking | ||
| LP 505: long pass 505 nm | ||
| PMT 3 | ||
| LP 560: long pass 560 nm | ||
| BP 500/20 IR: band pass 500 to 520 nm, IR-blocking | ||
| BP 500-550 IR: band pass 500 to 550 nm, IR-blocking | ||
| BP 535-590 IR: band pass 535 to 590 nm, IR-blocking | ||
| BP 565-615 IR: band pass 565 to 515 nm, IR-blocking | ||
| Detectors | ||
| Two photo-multiplier tubes fluorescence, 8 or 12 bit sampling (256 or 4096 gray levels, respectively) | ||
| One transmission detector for transmission / DIC imaging | ||
| One non-descanned detector for high-sensitivity (deep tissue) imaging | ||
| One Meta detector for spectral imaging. Up to 8 channels grouping any of the 32 detectors (10 nm each) may be defined (overlap between channels is not allowed). The Meta detector is almost as sensitive as a PMT except in the red, but twice as noisy. | ||
| Computer & Software | ||
| Fujitsu Siemens 2.4 GHz Xenon CPU running Windows 2000, 3.5 GB RAM, dual 114 GB storage, 2.4x DVD+/- recorder, dual 19" 1280 x 1024 flat panels | ||
| LSM 5 acquisition software with Physiology and Time Series modules | ||
